ncRNA name | NLRP3 siRNA |
ncRNA Category | siRNA |
Disease name | age-related macular degeneration (AMD) |
Species | Homo sapiens |
Tissues/Cell_line | human RPE cell line ARPE-19, primary adult hRPE cells |
Methods | siRNA transfection, immunohistochemistry, qRT-PCR, ELISA, Western blot, Transmission Electron Microscopy (TEM) |
Expression pattern | associated |
Functional description | In patients with AMD, we observed increased mRNA levels of NLRP3, pro-interleukin-1 beta (IL-1β) and pro-IL-18 in AMD lesions of the retinal pigment epithelium (RPE) and photoreceptor. In vitro, a similar increase was evoked by oxidative stress or lipopolysaccharide (LPS) stimulation in the adult retinal pigment epithelium (ARPE-19) cell line, and the increase was reduced in siRNA transfected cells to knockdown NLRP3. |
PubMed ID | 26760997 |
Year | 2016 |
Title | NLRP3 Upregulation in Retinal Pigment Epithelium in Age-Related Macular Degeneration. |
Drug-related ncRNA | NO |
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